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Abstract
Research of fed batch saccharification using paper mill primary sludge from Waste Water Treatment Plant (WWTP) has been conducted. The research was conducted in several stages i.e. sludge characterization, determining optimum dosage of cellulase and glucosidase through batch saccharification, and continued with fed batch saccharification. The characterization was performed on 3 primary sludge type from 3 paper mill, which are print paper mill with virgin pulp raw material, paper tissue mill with virgin pulp raw material and core board mill with waste paper raw material.
The characterization results showed that the sludge derived from the tissue paper mill with virgin pulp raw material has the highest content of cellulose which is 47.06%, this sludge was used as further research material. The initial trial of batch saccharification at 6% w/w and temperature of 60oC gave optimum dosage of cellulase 9 FPU/g cellulose and β-glucosidase 80 IU/g cellulose with reducing sugar 20.01 g/L and a saccharification efficiency of 63.84%.
Fed batch saccharification was performed on tissue paper mill sludge at cellulase and glucosidase enzyme doses respectively 9 FPU/g cellulose and 80 IU/g, temperature 60°C and initial pH of 4 with two variables, which are cumulative solid content (10%, 14%, and 18% w/v) and feed enzyme system (at the beginning and in step). The best results were obtained at fed batch sakarifikasi with gradual enzyme feeding at 14% solids content resulting in reducing sugar 43.899 g/L and efficiency 60.03%.
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Alda, A.G.O. De (2008) ‘Resources, Conservation and Recycling Feasibility of recycling pulp and paper mill sludge in the paper and board industries’, Resources, Conservation, and Recycling, 52(L), pp. 965–972. crossref
Alkasrawi, M., Al-Hamamre, Z., Al-Shannag, M., Abedin, M.J., Singsaas, E. (2016) ‘Conversion of paper mill residuals to fermentable sugars’, BioResources, 11, pp. 2287–2296.
Anindyawati, T. (2009) ‘Prospek enzim dan limbah lignoselulosa untuk produksi bioetanol’, Berita Selulosa, 44(Juni 1999), pp. 49–56.
Elliston, A., Collins, S.R.A., Wilson, D.R., Roberts, I.N., Waldron, K.W. (2013) ‘High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper’, Bioresource Technology. Elsevier Ltd, 134, pp. 117–126. crossref
Elliston, A., Collins, S.R.A., Faulds, C.B., Roberts, I.N., Waldron, K.W. (2014) ‘Biorefining of waste paper biomass: Increasing the concentration of glucose by optimising enzymatic hydrolysis’, Applied Biochemistry and Biotechnology, 172(7), pp. 3621–3634.
Fan, Z., Lynd, Æ.L.R. (2007) ‘Conversion of paper sludge to ethanol. I : Impact of feeding frequency and mixing energy characterization’, pp. 27–34. crossref
Gao, Y., Xu, J., Yuan, Z., Zhang, Y., Liang, C., Liu, Y. (2014) ‘Ethanol Production from High Solids Loading of Alkali - Pretreated Sugarcane Bagasse with an SSF Process’, BioResources, 9(2), pp. 3466–3479.
Harmsen, P.F.H., Huijgen, W., Bermudez, L., Bakker, R. (2010) 'Literature Review of Physical and Chemical Pretreatment Processes for Lignocellulosic Biomass', Food and Biobased Research. crossref
Howard, R.L., Abotsi E., Jansen van Rensburg, E.L., Howard, S. (2003) ‘Lignocellulose biotechnology: issues of bioconversion and enzyme production’, African Journal of Biotechnology, 2(12), pp. 602–619. crossref
Kang, L., Wang, W., Pallapolu, V.R., Lee, Y.Y. (2011) ‘Enhanced ethanol production from de-ashed paper sludge by simultaneous saccharification and fermentation and simultaneous saccharification and Co-Fermentation’, BioResources, 6(4), pp. 3791–3808.
Kristensen, J.B., Felby, C., Jørgensen, H. (2009) ‘Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose. ’, Biotechnology for biofuels, 2(1), p. 11. crossref
Lark, N., Xia, Y., Qin, C.G., Gong, C.S., Tsao, G.T. (1997) ‘Production of ethanol from recycled paper sludge using cellulase and yeast, kluveromyces markianus’, Biomass and Bioenergy, 12 (No. 2), pp. 135–143.
Lynd, L.R., Lyford, K., South, C.R., van Walsum, P.G., Levenson, K. (2001) ‘Evaluation of paper sludge for amenability to enzymatic hydrolysis and conversion to ethanol’, Tappi Journal Peer Reviewed Paper, 84 (February), pp. 50–55.
Marques, S., Alves, L., Roseiro, J.C., Girio, F.M. (2008) ‘Conversion of recycled paper sludge to ethanol by SHF and SSF using Pichia stipitis’, Biomass and Bioenergy, 32(5), pp. 400–406. crossref
Mussatto, S.I., Fernandes, M., Milagres, A.M.F., Roberto, I.C. (2008) ‘Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer’s spent grain’, Enzyme and Microbial Technology, 43(2), pp. 124–129. crossref
Olofsson, K., Palmqvist, B., Lidén, G. (2010) ‘Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding’, Biotechnology for biofuels, 3, p. 17. crossref
Peng, L., Chen, Y. (2011) ‘Conversion of paper sludge to ethanol by separate hydrolysis and fermentation (SHF) using Saccharomyces cerevisiae’, Biomass and Bioenergy, 35(4), pp. 1600–1606. crossref
Prasetyo, J., Naruse, K., Kato, T., Boonchird, C., Harashima, S., Park, E.Y. (2011) ‘Bioconversion of paper sludge to biofuel by simultaneous saccharification and fermentation using a cellulase of paper sludge origin and thermotolerant Saccharomyces cerevisiaeTJ14’, Biotechnology for Biofuels, 4(1), pp. 1–13. crossref
Saepulloh, Soetopo, R.S., Asthary, P.B. (2012) ‘Lumpur primer IPAL industri kertas sebagai bahan baku alternatif bioetanol’, Jurnal Selulosa. crossref
Shen, J., Agblevor, F.A. (2011) ‘Ethanol production of semi-simultaneous saccharification and fermentation from mixture of cotton gin waste and recycled paper sludge’, Bioprocess and Biosystems Engineering, 34(1), pp. 33–43. crossref
Soetopo, R.S., Purwati, S., Setiawan, Y., Sugesty, S. (2012) ‘Pemanfaatan Sludge Instalasi Pengolahan Air Limbah Industri Pulp Dan Kertas Sebagai Bahan Baku Bioetanol’, Jurnal Selulosa, 2(2), pp. 61–70. crossref
Tejirian, A., Xu, F. (2010) ‘Inhibition of cellulase-catalyzed lignocellulosic hydrolysis by iron and oxidative metal ions and complexes’, Applied and Environmental Microbiology, 76(23), pp. 7673–7682. crossref
Wang, W., Kang, L., Lee, Y.Y. (2010) ‘Production of cellulase from kraft paper mill sludge by trichoderma reesei rut C-30’, Applied Biochemistry and Biotechnology, 161(1–8), pp. 382–394. crossref
Ximenes, E., Kim, Y., Mosier, N., Dien, B., Ladisch, M. (2011) ‘Deactivation of cellulases by phenols’, Enzyme and microbial technology. Elsevier Inc., 48(1), pp. 54–60. crossref
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