Toksisitas Subkronis Timbal (Pb) dalam Limbah Padat IPAL Industri Kertas terhadap Karakteristik Darah Tikus Rattus norvegicus

Rina S Soetopo; Ahmad Ridwan; Suripto Suripto

doi:10.25269/jsel.v40i2.246

Toksisitas Subkronis Timbal (Pb) dalam Limbah Padat IPAL Industri Kertas terhadap Karakteristik Darah Tikus Rattus norvegicus

Rina S Soetopo^(1*), Ahmad Ridwan⁽²⁾, Suripto Suripto⁽³⁾
(1) Center for Pulp and Paper, Ministry of Industry
(2) Department of Biology, Institut Teknologi Bandung
(3) Department of Biology, Institut Teknologi Bandung
(*) Corresponding Author
DOI: http://dx.doi.org/10.25269/jsel.v40i2.246

Abstract

The primary environmental problem associated with open dumping solid waste disposal is the leachate potency to contaminates ground and surface waters. Paper industry solid waste using mixture raw material with deinked pulp is contaminated by heavy metals. One of the heavy metals is lead (Pb) which is dominant in waste. The effects of subchronic toxicity of paper solid waste to blood characteristics of rat (Rattus norvegicus) have been done. Solid waste extract was administered to each individual rat by gavage through gastrointestinal tract at three different doses (350; 2500 and 5000 mg/kg body weight/day) and also positif control (0,5; 5,0; 50; 500; 5000 µg/kg body weight/day) and negatif control (water) daily during 90 days period. Results showed that subchronic toxicity effect of lead in 5000 μg/kg body weight/day of paper industry solid waste caused reduction to blood δ-ALA-D activities, hemoglobin concentration and hematocrit value, but it was still in normal ranges.

Keywords

solid waste; blood ALA-D activities; blood characteristic; blood lead

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References

Agency for Toxic and Disease Registry (ATSDR). 1999. Toxicology Profile for Lead. Public Health Service, U.S. Department of health and Human Service. Atlanta.

Campbell, N.A., Reece, J.B. & Mitchell, L.G. 1999. Biology. Fifth edition. Benjamin Ocummings. San Francisco. 3. Campbell, N.A., Mitchell, L.G. & Reece, J.B. 2000. Biology Concepts and Connections.

Addison Wesley Longman, Inc. San Francisco.

Connell, D.W. & Miller, G.J. 1995. Chemistry and Ecotoxicology of Pollutan. Willey Interscience Publication. 342-386. 5. Ferguson, K. 1991. Environmental Solution for the Pulp and Paper Industry. Miller

Freeman. San Fransisco. 171-176.

Forbes, V.E. & Forbes, T.L. 1994. Ecotoxicology in Theory and Practice. Chapman & hall. London. 110-112.

Goyer, R.A., Kuhn, C. & Davis, G.L. 1975. Environmental Pathology. Academic Press. New York. 23-29.

Grandjean, P. 1984. Biological Effects of Organolead Compounds. Florida CRC Press. 98-100.

Hodson, P.V., Blunt, B.R., Spry, D.J. & Austen, K.1977. Evaluation of Erythrocyte δ - Aminolevulinic Dehydratase Activity as a Short-Term Indicator in Fish of a Harmful Exposure to Lead. J.Fish.Res. Board Can. 34: 501-508.

Kelada, S., Shelton, E., Kaufmann, R.B. & Khoury, J.K. 2001. δ-Aminolevulinic Acid Dehydratase genotype and lead toxicity. American Journal of Epidemiology. 154 (1): 1-13.

Klassen, C.D. 2001. Toxicology The Basic Science of Poisons. 6thed. The McGraw-Hill Companies. USA. 827-833.

Lu, F.C. 1995. Basic Toxicology, Fundamentals, target organs and Risk assesment. Hemisphere publishing corporation. 346-370.

Martini, F.H. 1998. Fundamentals of Anatomy and Physiology. Fouth Edition. Prentice Hall, Inc. New Jersey. 647-650.

Mugahi, M.N. & Heidari, Z. 2003. Effects of Chronic Lead Acetate Intoxication on Blood Indices of Male Adult Rat. DARU. 11(4): 147-150.

Mushak, P. 1991. Gastro-intestinal Absorption of Lead in Children and Adults. Overview of Biological and Biophysico-chemical Aspects.

Chemical Speciation and Bioavailability 3: 87- 104.

Mutschler, E. 1999. Dinamika obat. Farmakologi dan Toksikologi (terjemahan). Edisi ke 5. Penerbit ITB. Bandung. 700-740.

Nakagawa, H., Sato, T & Kubo, H. 1995. Method Not Requiring Mercuric Chloride for Determination of Activity of δ-Aminolevulinic

Acid Dehydratase in Blood of Carp Cyprinus carpio. Fish.Sci. 61(1): 97-99.

Organization for Economic Cooperation and Development (OECD) TG-408. 1998. Sub Chronic Oral Toxicity Test-Repeated Dose 90-day Toxicity Study in Rodents.

Purwati, S. Rina. S. Soetopo. 2001. Kajian Karakteristik Lumpur IPAL-IPK berkaitan Dengan Peraturan Pengelolaan Limbah B-3. Prosiding Seminar Teknologi Selulosa. Balai Besar Penelitian dan Pengembangan Industri Selulosa. Bandung. 85-95.

Rober, D.R. 2000. Toxicology of Heavy Metals. Departemen of Chemical Pathology. The Chinese university of Hongkong.

Robinowitz, M.B. & Wettherill, G.W., & Kopple, J.D. 1976. Kinetic Analysis of Lead Metabolisme in Healthy Human. J.Lab.Clin

Inves. 58. 265-270.

Richardson et al., 1992, The environmental impact of deinking : a pilot study, APPITA, Vol. 45 No. 5, 314 - 318.

Ruslijanto, H. 1984. Keracunan timah hitam, sumber-sumber, bahayanya dan penanggulangannya. Majalah Ilmiah Kedokteran Gigi.

Universitas Trisakti. Jakarta . Sakai, T. 2000.

Biomakers of lead Exposure. Industrial Health. 38: 127-142.

Santoso, S. 2004. SPSS Statistik Parametrik. Edisi ke 4. PT Elex media komputindo. Gramedia. Jakarta.

Schiefer, B., Donald, G.I & Buzik S. C. 1987. You and Toxicology. Toxicology Research Center University of Saskatchewan. Apex Graphics Saskatoon. 5 - 12.

Sipos, P., Szentmihalyi, K., Feher, E., Abaza, M., Szilagyi, M. 2003. Some Effects of Lead Contamination on Liver and Gallbladder Bile. Acta Biologica Szegediensis. 47 (1-4): 139-142.

Toro, G & Ackermann, P.G. 1975. Practical Clinical Chemistry. Little, Brown and Company. Boston. 539-541.

U.S. Environmental Protection Agency (USEPA), 1996. Technology Transfer Handbook Management of water Treatment Plant Residuals. American Water Works Association. Denver. Colorado. 125-136.

Ziegler, E.E., Edwards, B.B., Jensen, R.L. 1978. Absorption of Retention of Lead by Infants. Pediatr. Res 12: 29-34.

Zutphen, L.F.M., Baumans, V. & Beynen, A.C. 1993. Principles of Laboratory Animal Science. Elsevier. Amsterdam. 17-20.

DOI: http://dx.doi.org/10.25269/jsel.v40i2.246